High-Throughput Screening (HTS)
We perform assay development and high-throughput screening of a wide variety of targets, such as enzymes (including e.g. kinases, proteases and epigenetic targets), GPCRs, nuclear hormone receptors and ion channels.
iNovacia's basic HTS features include:
- Development of new biochemical and cellular assays
- Efficient transfer and miniaturization of our
client assay - Cost effective and flexible screening setup solutions
- Biophysical characterization of the protein utilized in the assay setup
- Routine in performing a large variety of assay types including isotopic assays
- All data in Electronic Lab Notebooks
A good assay must be sensitive, robust and cheap. It also needs to maximize the chances to detect hit compounds while avoiding false positives and negatives. Therefore, we design the right assay to discover the right type of compounds: antagonists and agonists (full, partial), inhibitors (reversible, slow binders, tight binders), and allosteric modulators.
Screening is typically performed in 384-well format for both biochemical and cell-based assays. We can use a broad range of assay technologies like absorbance, luminescence, fluorescence intensity, fluorescence polarization (FP), fluorescence resonance energy transfer (FRET), AlphaScreen, time resolved fluorescence (DELFIA, LANCE, HTRF), fluorescence kinetics and scintillation proximity (SPA). In addition, we have good experience of more difficult and/or specific assays including butanol extraction, separation after charcoal filtration and 14CO2 evaporation. After the screening, we confirm the primary hits by dose-response curve analysis accompanied by identity, purity, stability and solubility measurements.
